I am interested in the conformational dynamics of biomolecular systems. I have been using both ensemble- & single-molecule FRET to study systems such as Adenylate Kinase, the B-domain of protein A, the bacterial transcription initiation complex, and recently early oligomers of alpha-synuclein.
In recent years, I have been heavily involved in developments of open science practices in FRET. These include: open source-code software tools organized on the open single-molecule fluorescence spectroscopy project (openSMFS; initiated in the Weiss lab), promoting data reproducibility through deposition of raw data and full analysis documentation, preparing Jupyter notebooks including full recipes for common tasks for the use of others, etc. I intend to keep on helping bridge the gap between many practitioners as well as newbies to FRET, through promotion of open science practices, open data & open code. With that, I hope we will be able to form a unite community.
Selected Publications:
Ploetz E, Ambrose B, Barth A, Börner R, Erichson F, Kapanidis AN, Kim HD, Levitus M, Lohman TM, Mazumder A, Rueda DS, Steffen FD, Cordes T, Magennis SW, Lerner E. (2023). A new twist on PIFE: photoisomerisation-related fluorescence enhancement. Methods and Applications in Fluorescence. 12(1):012001
Joron K, Viegas JO, Haas-Neill L, Bier S, Drori P, Dvir S, Lim PSL, Rauscher S, Meshorer E, Lerner E. (2023). Nature Communications. 14: 4885
Zamel J, Chen J, Zaer S, Harris PD, Drori P, Lebendiker M, Kalisman N, Dokholyan NV, Lerner E. (2023). Structural and dynamic insights into α-synuclein dimer conformations. Structure. 31(4):411.
Harris PD, Lerner E. (2022). Identification and Quantification of Within-Burst Dynamics in Singly-Labeled Single-Molecule Fluorescence Lifetime Experiments. Biophysical Reports. 2(3): 100071
Harris PD, Narducci A, Gebhardt C, Cordes T, Weiss S, Lerner E. (2022). Multi-parameter photon-by-photon hidden Markov modeling. Nature Communications. 13:1000
Ingargiola A, Weiss S, Lerner E. (2018). Monte-Carlo Diffusion-Enhanced Photon Inference: distance distributions and conformational dynamics in single-molecule FRET. J. Phys Chem. B. 122(49): 11598-11615
Lerner E, Cordes T*, Ingargiola A, Alhadid Y, Chung S, Michalet X, Weiss S. (2018). Towards dynamic structural biology: Two decades of single-molecule Förster resonance energy transfer. Science. 359(6373): eaan1133
Lerner E, Ingargiola A, Weiss S. (2017). Characterizing highly dynamic conformational states: the transcription bubble in RNAP-promoter open complex as an example. J. Chem. Phys. 148(12):123315
Lerner E, Chung S*, Allen BL, Wang S, Lee JJ, Lu WS, Grimaud LW, Ingargiola A, Michalet X, Alhadid Y, Borukhov S, Strick T, Taatjes DJ, Weiss S. (2016). A backtracked and paused transcription initiation intermediate of Escherichia Coli RNA polymerase. Proc Natl Acad Sci U S A. 113(43):E6562-E6571
Ploetz E, Lerner E, Husada F, Roelfs M, Chung S, Hohlbein J, Weiss S, Cordes T. (2016). Förster resonance energy transfer and protein-induced fluorescence enhancement as synergetic multi-scale molecular rulers. Scientific Reports. 6:33257
Ingargiola A, Lerner E, Chung S, Weiss S, Michalet X. (2016). FRETBursts: An Open Source Toolkit for Analysis of Freely-Diffusing Single-Molecule FRET. PLoS One 11(8):e0160716
Lerner E, Ploetz E*, Hohlbein J, Cordes T, Weiss S. (2016). A Quantitative Theoretical Framework for Protein-Induced Fluorescence Enhancement-Forster-Type Resonance Energy Transfer (PIFE-FRET). J. Phys Chem. B. 120(26):6401-10
